Hcs lipidtox
WebHCS LipidTOX Red solution from the manufacturer µl/10 ml PBS). Cells in MatTek dishes were stained after fixation by addition of 2 ml solution of dye in PBS. To reduce the effects of temperature variation on stain-ing efficiency, we incubated the fixed cells in staining solution for 30 min at 37C. They were then immedi- WebExcess lipid accumulates in vesicles that displace the cytoplasm; large accumulations of lipids can disrupt cell constituents, and in severe cases, the cell may even burst. Steatosis is a common consequence of hepatocellular insult, which can be drug-induced or can be an outcome in pathophysiogical disease progression.
Hcs lipidtox
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WebAug 20, 2024 · To better characterize a potential role of TMEM41B in regulating LDs, we analyzed H4 cells basally or upon treatment with BSA-conjugated oleic acid by probing with HCS LipidTox Green Neutral Lipid Stain, a probe with high affinity for neutral LDs. WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and …
WebSep 26, 2024 · For time-lapse imaging, live cells were treated with different agents and HCS LipidTOX red neutral lipid dye (Invitrogen, 1:200) was immediately added. ... LipidTOX staining confirmed the increase in lipid-droplet accumulation in these cells (Fig. 6E, F). Thus, BMP2 is a positive regulator of lipogenic differentiation in human IPF lung ... WebCitations & References The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid …
WebOct 30, 2024 · However, the combination of HCS LipidTOX™ steatosis reagents provides a labeling strategy to perform high content imaging multiplexed assays to characterize phospolipidosis and steatosis, and to measure the effects on lipid metabolism that can be triggered by drugs or other compounds. WebJan 1, 2014 · HCS LipidTOX Red staining. This section describes cell staining with HCS LipidTOX Red (Invitrogen). Alternative colors of the same reagent and similar reagents from other sources are available and should work just as well. HCS LipidTOX Red can also be applied to live cell imaging. 1. Fix cells (see Section 3.5) and remove the PBS. 2.
WebDec 7, 2024 · Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP). Results: The ethanol extraction process aggravated the hepatotoxicity of …
WebThermo Fisher hcs lipidtox green neutral lipid stain solution Hcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more boise lawn mowingWebHCS LipidTOX™ phospholipidosis detec-tion reagents can be easily detected with fluorescence microscopes or HCS readers equipped with standard filter sets (Figure … glow under snowboardWebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. Next, the formaldehyde was removed, and the cells were gently … boiselecWebApr 28, 2016 · 2μL ethidium homodimer (LIVE/DEAD Viability/Cytotoxicity Kit; Molecular Probes) and 1μL of HCS LipidTOX Deep Red (Molecular Probes) was mixed into each well containing placental explants. After 30mins, 100μL of 10μM BODIPY-C 12 solution was added to each well and mixed by trituration (total well volume 500μL, final concentration … glow under eye concealerWebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is … glow ufoWebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. Description; Category: Stain; MW: N/A. Stock Concentration: 1000 X. Preparation; Solvent for Stock Solution: DMSO. Storage; Temperature-20°C; Precaution(s) Protected from light; glow unitframe on debuff weakauraWebNov 18, 2024 · WT and SKO HeLa cells expressing myc-DFCP1 and mCherry-EV or mCherry-seipin were treated with 400 µM oleate for 6 h, fixed, and stained with anti-myc antisera. LDs were then stained with HCS LipidTOX Deep Red Neutral Lipid Stain (purple). glowuniverse.com reviews