WebApr 12, 2024 · Bob Rice. First of all, a huge THANK YOU to all who supported the fundraising efforts for “The Hours.”. WE DID IT! Actually, we more than did it. 64 backers pledged … Arabidopsis thaliana seeds were stratified at 4 °C for 2 days and sown in soil. Plants were grown in a controlled environmental chamber under the following conditions: 23 °C, 70% relative humidity, a 10-h light/14-h dark photoperiod, and a light intensity of 85 µE/m2/s. N. benthamiana plants were grown in the growth … See more A. thaliana ecotype Columbia (Col-0) was used as the WT. Arabidopsis T-DNA insertion lines in the Col-0 background for pire-1 (SALK_079510) and pire-2 … See more For co-immunoprecipitation in N. benthamiana, pENTR-LTI6b-3xFLAG and pENTR-RBOHD were recombined into pEarlygate104 to generate YFP-tagged fusions. … See more The N-terminus (aa1–376) and the C-terminus (aa756–912) of RBOHD cDNA was PCR amplified and directionally cloned into pENTR/D-TOPO (Invitrogen). The … See more In vitro kinase assays were performed as described in previously25. 0.5 μg of purified HIS-PBL13, HIS-BIK1, and HIS-PBL13ΔC and 2 μg of HIS-RBOHD-C were … See more

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